Signalling

Part:BBa_I765013:Design

Designed by: Juliana Maria Navia and Alex Quintero   Group: iGEM07_Colombia_Israel   (2007-10-26)

Iron promoter- luxI HSL signaling device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 160
    Illegal PstI site found at 712
    Illegal PstI site found at 940
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 160
    Illegal PstI site found at 712
    Illegal PstI site found at 940
    Illegal PstI site found at 961
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1707
    Illegal XhoI site found at 814
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 160
    Illegal PstI site found at 712
    Illegal PstI site found at 940
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 160
    Illegal PstI site found at 712
    Illegal PstI site found at 940
    Illegal PstI site found at 961
    Illegal NgoMIV site found at 47
    Illegal NgoMIV site found at 397
    Illegal NgoMIV site found at 511
    Illegal NgoMIV site found at 565
    Illegal NgoMIV site found at 868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 421
    Illegal BsaI.rc site found at 1856


Design Notes

this device is composed by BBa_I765000 and BBa_S01983 it comes in pSB1A2


Source

genomic sequence

References